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Diff Quik (DQ) stains are commonly used in practice to examine blood smears and cytology samples. It is a three-step system using methanol as a fixative Tap gently on absorbent paper towel to remove access stain. 5. Rinse: Gently run water over the slide to rinse off the access stain. 6. Prop slide upright 1. Bring sections to distilled water · 2. Stain with “Diff Quick” solution II .30 secs · 3. Counterstain (optional) with “Diff Quick” solution I for 30 seconds · 4 It is a valuable stain for blood cell differential count and evaluations. It also works nicely for general diagnostic cytology. DIRECTIONS FOR USE. 1. The slideThe Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). RAL Diff-Quik kit is a fast-acting variation of Romanowsky staining. Take 2 µl of blood out of an EDTA tube and place them in the center of a slide.