Amicon ultra 10k manual

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Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8. Typical Concentrate Volume/Concentration Factor vs. Spin Time 3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL). Typical Protein Recovery. Membrane NMWL, kDa, Protein Solute, Protein The Amicon® Ultra-4 10K device is supplied with a cap, a filter device, and a centrifuge tube. Assemble device and centrifuge per instructions. Page 1 User Guide ® Amicon Ultra-4 Centrifugal Filter Devices for volumes up to 4 mL Amicon Ultra-4 3K, 10K, 30K, 50K, and 100K devices for research use only; Amicon​​Ultra-15​​10K. Centrifugal​​Filter. Aim. Centrifuge​​away​​particles​​smaller​​than​​10​​kDa​​or​​perform​​a​​buffer​​exchange. Procedure. Rinsing. Amicon Ultra-0.5 filters to concentrate your sample in the Amicon Ultra-0.5 filter, turn and spun according to the manufacturer's instructions.Amicon Ultra ultrafiltration device combines Ultracel™ volume, spin times were 10 minutes for 10K and 30K membranes, and 15 minutes for 100K membranes. Refer to the Amicon® Ultra-0.5 User Guide for further information. NOTE: For antibody labeling, we recommend using the Amicon® Ultra-0.5 10K device. Amicon® Ultra-0.5. Centrifugal Filter Devices for volumes up to 500 µL. User Guide. For research use only; not for use in diagnostic procedures. 10K.